Metabolic Pathway Characterization from Transient Response Data Obtained In Situ: Parameter Estimation in S-system Models
✍ Scribed by Albert Sorribas; Salvador Samitier; Enric I. Canela; Marta Cascante
- Book ID
- 102975722
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 725 KB
- Volume
- 162
- Category
- Article
- ISSN
- 0022-5193
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✦ Synopsis
The actual values of internal metabolites and fluxes can be measured by a number of experimental techniques and they provide important information for evaluating the properties of a metabolic pathway in situ. In this paper we propose a strategy to properly exploit this information. The suggested approach permits estimation of a set of parameters on the whole system so that a useful model can be constructed and used to describe its components and systemic properties and to predict its behavior under new conditions. A simulated reference pathway is provided to validate this method and to show its utility in metabolic studies.