Metabolic formation of dodecanedioic acid from n-dodecane by a mutant ofCandida tropicalis

✍ Scribed by Zu-Hua Yi; Hans-Jürgen Rehm

• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 373 KB
• Volume: 14
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00498473

✦ Synopsis

These experiments studied the metabolic formation of a,co-dodecanedioic acid using the mutant S 76 developed from the wild strain Candida tropicalis 1230 (capable of producing large amounts of a,w-dodecanedioic acid).

Our results show for the first time that 12-hydroxydodecanoic acid was excreted into the medium as a free acid.

n-Dodecanol and n-dodecanoic acid were also detected in the n-dodecane medium. The mutant S 76 was able to produce a, co-dodecanedioic acid using either n-dodecanol or dodecanoic acid as the sole carbon source. Quantitative cahnges in the concentrations of 12-hydroxy-dodecanoic acid and other intermediates were recorded during the formation of a, co-dodecanedioic acid. S 76 was rapidly able to convert large amounts of 12-hydroxy-dodecanoic acid to a,co-dodecanedioic acid.

The formation of a,co-dodecanedioic acid from n-dodecane via the sequence n-dodecanol -+ n-dodecanoic acid -+ 12-hydroxy-dodecanoic acid was confirmed.