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Metabolic flux-based optimisation of recombinant human interleukin-3 expression by Streptomyces lividans 66

โœ Scribed by Keyvan Nowruzi; Ali Elkamel; Jeno M. Scharer; Douglas Cossar; Murray Moo-Young


Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
247 KB
Volume
89
Category
Article
ISSN
0008-4034

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Development of a minimal defined medium
โœ Keyvan Nowruzi; Ali Elkamel; Jeno M. Scharer; Doug Cossar; Murray Moo-Young ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 193 KB ๐Ÿ‘ 2 views

## Abstract A systematic approach was developed to identify and optimize the essential amino acids in defined minimal medium for the production of recombinant human interleukin 3 (rHuILโ€3) by __Streptomyces lividans__. Starvation trials were carried out initially to narrow down the number of probab