Metabolic engineering of Clostridium acetobutylicum for the production of butanol at high yield
β Scribed by P. Soucaille
- Publisher
- Elsevier
- Year
- 2009
- Tongue
- English
- Weight
- 66 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1871-6784
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π SIMILAR VOLUMES
Low volumetric solvent productivities are one of the characteristics of an acetone-butanol fermentation by C. acetobutylicum. A calcium alginate immobilized continuous culture was used in a novel gas-sparged reactor to strip the solvents from the aqueous phase and reduce their toxicity. A dilution r
Metabolic flux analysis was used to investigate the roles of the acid formation pathways in Clostridium acetobutylicum. The acid formation pathways were revealed to serve different roles in wildtype fermentations than previously expected. Specifically, enzymes known to catalyze butyrate formation we
Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, whi