<span>This edited book highlights the plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of inter
Metabolic Engineering in Plants: Fundamentals and Applications
β Scribed by Tariq Aftab (editor), Khalid Rehman Hakeem (editor)
- Publisher
- Springer
- Year
- 2022
- Tongue
- English
- Leaves
- 414
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
This edited book highlights the plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species.
Metabolic engineering is usually defined as the re-routing of one or more enzymatic reactions to generate new compounds, increase the production of existing compounds, or facilitate the degradation of compounds. Plants are the foundation of numerous compounds which are synthesized via assimilated complex biosynthetic routes. Plants have evolved an incredible arrangement of metabolic pathways leading to molecules/compounds capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. However, in foreseeable future we will be forced to think about the accessibility of resources for the generations to come. For these reasons, the book proposes alternative options of food/food supplement, medicines and other essential items, by using plant metabolic engineering approach. This book is of interest to teachers, researchers and academic experts. Also, the book serves as additional reading material for undergraduate and graduate students of biotechnology and molecular biology of plants.β¦ Table of Contents
Preface
Contents
About the Editors
Chapter 1: Metabolic Engineering: New Approaches in Pharmaceutical Production
Introduction
Strategies
Host Selection
Examples of Engineered Metabolites
Taxol (TXL)
Biosynthetic Background
Conventional Strategies to Increase TXL Yields
Biotechnological Strategies
Cell Culture
Heterologous Expression Systems
Overexpression Positive Effectors
Knock-Outing Negative Effectors
Camptothecin (CPT)
Biosynthetic Background
Hairy Root Culture
Endophytes
Vinca Alkaloids (Vincristine (VCT) and Vinblastine (VBL)
Biosynthetic Background
Artemisinin (ATN)
Biosynthetic Background
Heterologous Expression Systems
Overexpression of Key Biosynthetic Genes
Silymarin
Conclusion
References
Chapter 2: Ameliorating Abiotic Stress Tolerance in Crop Plants by Metabolic Engineering
Introduction
Abiotic Distress in Crop Plants
Mediators of Plant Responses Towards Abiotic Stresses
Osmoprotectants
Polyamines
Flavonoids
Phytohormones
Metabolic Engineering of Biosynthates for Promoting Plant Tolerance Towards Abiotic Stress
Conclusions
References
Chapter 3: Enhancing Photosynthetic Efficiency of Crop Through Metabolic Engineering
Introduction
Photosynthetic Efficiency of Crop and WUE
The Efficiency of Photosynthesis Genetic Engineering
Engineering Photosynthesis: Progress and Prospects
Engineering Rubisco
Calvin-Benson Cycle Optimization
Photosynthesis and Crop Yield
Current and Possible Approaches for Improving Photosynthetic Efficiency
Plant Productivity Depends on Many Genetic
Photosynthetic Metabolism as a Basis for Crop Production and Yield Improvement Under Stressful Conditions
Photosynthetic Metabolism in a Changing Environment
Environmental Factors
The Effect of Abiotic Stress on Plants
High Temperature
Water Stress
Plant Hormones and Interrelated Genetic and Biochemical Mechanisms
Salt Stress Significantly Affects Photosynthesis
The Influence of Heavy Metals
Conclusions and Further Opportunities
References
Chapter 4: Transcription Factor Mediated Plant Metabolite Production in Response to Environmental Stress Factors: Current Unde...
Introduction
Transcription Factors in the Regulation of Secondary Metabolite Gene Expression Under Abiotic Stress
WRKY Transcription Factors
bHLH Transcription Factors
bZIP Transcription Factors
NAC Transcription Factors
AP2/ERF Transcription Factor
MYB Transcription Factors
Effective Manipulation of Secondary Metabolite Pathways by Transcription Factors
Synthetic Promoters and Synthetic TF-Mediated Metabolite Engineering
Synthetic Promoters
Synthetic Transcription Factors
Conclusion and Future Aspects
References
Chapter 5: Secondary Metabolite Engineering for Plant Immunity Against Various Pathogens
Introduction
Phytoanticipins and Phytoalexins
Metabolic Engineering and Infection Control
Engineering Pathogen Resistance
Isoflavones
Hydroxycinnamic Acid Amides
Terpenoids
Camalexin
Alkaloids
Mode of Action
Metabolic Engineering: Technical Challenges and Opportunities
Biosynthetic Gene Clusters and Gene Identity
The Regulation and Coordination of Gene Expression
Engineered Transportation and Metabolite Localization
New Technologies of Genome Editing
Trait Stacking
Position for Metabolic Engineering of Disease Resistance in Various Crops
Transgenic Crops
Conclusion
References
Chapter 6: Role of Metabolic Engineering in Enhancing Crop Nutritional Quality
Introduction
Approaches for Metabolic Engineering and Synthetic Biology in Plants
Strategies for Synthetic Metabolic Engineering
Strategies for Multigene Transformation
Identification of Genes with Potential to Improve the Nutritional Quality of Plants
Vitamin A
Vitamin E
Vitamin B6
Vitamin B9
Iron
Engineering Crops for Biofuel Production
Enhancing Photosynthetic Efficiency
Metabolic Engineering of Vitamin Stability in Crop Plants
Bioavailability of Vitamins in Biofortified Crop Products
Flavonoid and Anthocyanin Biofortification in Crops
Conclusion
References
Chapter 7: miRNA- and RNAi-Mediated Metabolic Engineering in Plants
Introduction
Biogenesis of microRNAs
Mode of Action of Plant miRNA
miRNA-Mediated Metabolic Engineering in Plants
Abiotic Stress-Resistant Crops
Secondary Metabolite (SM) Production
Conclusion
References
Chapter 8: CRISPR/Cas Genome Editing in Engineering Plant Secondary Metabolites of Therapeutic Benefits
Introduction
Background Study of CRISPR-Cas9
Secondary Metabolite Engineering of Plants with CRISPR/Cas9
Atropa belladonna L.
Brassica napus L.
Camelina sativa (L.) Crantz
Dendrobium officinale Kimura and Migo
Dioscorea zingiberensis C. H. Wright
Glycine max (L.) Merr.
Humulus lupulus L.
Nicotiana tabacum L.
Papaver somniferum L
Salvia miltiorrhiza Bunge
Challenges Associated with the Use of CRISPR/Cas Tools for Plant Natural Product Research
Off-Target Effects
Editing Accuracy
Efficient Delivery
Genome Instability
Toxicity of Cas9
Indigenous Vs Introduced CRISPRΒ΄s Role
Perspectives of CRISPR/Cas Genetic Engineering on Plant Natural Products
Conclusion
References
Chapter 9: Enhanced Production of Plant Aromatic Compounds Through Metabolic Engineering
Introduction
The Shikimate Pathway
3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase
3-Dehydroquinate Synthase (DHQS)
3-Dehydroquinate Dehydratase (DHD)-Shikimate Dehydrogenase (SDH)
Shikimate Kinase (SK)
5-Enolpyruvylshikimate 3-Phosphate (EPSP) Synthase
Chorismate Synthase (CS)
The Tryptophan Pathway
Anthranilate Synthase (AS)
Phosphoribosylanthranilate Transferase (PAT)
Phosphoribosylanthranilate Isomerase (PAI)
Indole-3-Glycerol Phosphate Synthase (IGPS)
Tryptophan Synthase (TS)
The Phenylalanine and Tyrosine Pathways
Chorismate Mutase (CM)
Prephenate Aminotransferase (PPA-AT)
Prephenate and Arogenate Dehydratases (PDT and ADT)
Arogenate and Prephenate Dehydrogenases (ADH and PDH)
Phenylpyruvate and 4-Hydroxyphenylpyruvate Aminotransferases (PPY-AT and HPP-AT)
Phenylalanine Hydroxylase
Regulation of Aromatic Amino Acid Biosynthesis
Transcriptional Regulation
Posttranscriptional Regulation
E4P and PEP Precursor Supply to the Shikimate Pathway
Subcellular Localization of Aromatic Amino Acid Biosynthesis in Plants
Transport of Aromatic Amino Acids and Pathway Intermediates across Plastid Membranes
Plant-Based Biosynthesis of Aromatic Amino Acid
References
Chapter 10: Molecular Farming: Sustainable Manufacturing of Vaccines, Antibodies, and Other Therapeutic Substances
Introduction
Plant-Derived Vaccines
Vaccine Antigens from Plant Suspension Cell Cultures
Vaccine Antigens from Hairy Root Cultures
Vaccine Antigens from Microalgae
Plant-Based Production Systems and Plant-Derived Protein Products
Plant-Derived Veterinary Vaccines
Therapeutical Applications in Terms of Health Supplements and Topical Utility
Plant Molecular Farming as a Strategy Against COVID -19
Neutralizing Antibodies
Other Proteins
Antigen-Based Vaccines
PMF Production Platforms
Transient Expression Platform
Bioreactor-Based Platforms
Plant-Cell-Culture System
Moss Culture
Algal Bioreactors
Seed-Based Platforms
Advantages
Limitations
Conclusion
References
Chapter 11: CRISPR/Cas-Mediated Genome Engineering for Abiotic Stress Resilience in Plants
Introduction
Cataloguing of CRISPR/Cas
Plant Genome Engineering with CRISPR/Cas Systems
CRISPR/Cas-Mediated Genome Engineering for Abiotic Stress Resilience in Plants
CRISPR/Cas9-Based Genome Editing (GE)
Zea Mays L.
Arabidopsis Thaliana L.
Oryza Sativa L.
Solanum Lycopersicum L.
Tetraselmis Suecica
Glycine max L.
Carica Papaya L.
Gossypium Spp.
Musa Acuminata Colla
CRISPR/Cas12-Based Genome Editing (GE)
Conclusion
References
Chapter 12: Manipulation of Key Genes Involved in Biosynthesis of Terpenoid Compounds in Plants
Introduction
Terpenoid Metabolic Engineering Approaches
Engineering Monoterpenes
Engineering Sesquiterpenoids
Engineering Diterpenoids
Engineering Triterpenoids
Engineering Tetraterpenoids
Potential Limitations and Future Prospects
Concluding Remarks
References
Chapter 13: Use of Metabolic Engineering/Biotechnology in Crops Breeding and Development of New Crops
Introduction
Methods of Plant Breeding
Conventional Methods
Genetic Engineering Methods
Approaches Used for the Development of Transgenic Crops
Various Application of Genetically Modified Crops
Nutritional Value
Omega-3 Oils
Essential Amino Acids
Photosynthetic Efficiency and Improved Yield
Delayed Ripening/Increased Shelf Life
Fungal Resistance
Herbicide Tolerance
Glyphosate
Yields of HR Crops
Edible Vaccines
Genes in Transgenic Plants that Confer Dehydration Stress Resistance
Enzymes that Synthesize Osmoprotectants
Mannitol
Proline
Glycine Betaine
Polyamines
Detoxification Enzymes or Oxidative Stress-Related Genes
Conclusion
References
Chapter 14: Improving the Quality of Medicinal and Aromatic Plants Through Metabolic Engineering
Introduction
Goals and Strategies of Metabolic Engineering
Metabolic Pathways of Interest
Recent Advancements in Metabolic Engineering of Plants
Secondary Metabolic Pathways
Shikimic Acid Pathway
Mevalonic Acid (MVA) Pathway
2-C-Methyl-d-Erythritol 4-Phosphate (MEP) Pathway
Utilization of Metabolic Engineering in the Manipulation of Secondary Metabolism
RNAi-Mediated Gene Silencing
RNAi for Disease Resistance in Plants
Case Studies
Alkaloids
Terpenoids
Flavonoids
Future Prospects
References
Chapter 15: Polymeric Composites: A Promising Tool for Enhancing Photosyntheticy Efficiency of Crops
Introduction
Role of Photosynthesis for Improving the Growth of the Plants
Effect of Metal-/Ceramic-Based Nanomaterials (MC-NMs) on the Photosynthesis
Effect of Carbon-Based Nanomaterials (CBNMs) on the Photosynthesis
Effect of Polymeric Composite on the Photosynthesis
Conclusion
References
Chapter 16: Metabolic Engineering Approaches to Produce Compounds of Interest in Plants
Introduction
Flavonoids
Alkaloids
Betalains
Glucosinolates
Gene Transfer and Expression Modification Method
Multigene Transformation Methods
Stacking and Retransformation
High-Capacity TDNA Vectors
TDNA and Bombardment Vectors
Split Reading Frames
Controlling the Expression of Multiple Transgenes
Combinatorial Transformation
Production of Pharmaceutically Relevant Molecules and Precursors
Vitamins
Metabolic Synthesis of Vitamin A
Metabolic Production of Vitamin D
Vitamin K Is Produced in the Body Through Metabolic Processes
Steroids
Unnatural Amino Acids
Metabolites of Pharmacological Significance
Strategies of Metabolic Engineering Used for Enhancement of Bioactive Compounds
Production of Bioactive Compounds from Cell Culture
Regulation of Biosynthesis Pathway Genes
Current Trends in Metabolic Engineering
References
Chapter 17: Carbon-Based Nanomaterials: An Efficient Tool for Improving the Nutritional Quality of Crops
Introduction
Role of Metabolic Engineering to Improve the Nutrition Quality of Plants
Role of NMs in Metabolic Engineering
Role of Carbon-Based Nanomaterials (CNMs) in Metabolic Engineering
Interaction of CNMs with Plant
Conclusion
References
Chapter 18: Plant Metabolic Engineering for a Futuristic Economy
Introduction
Strategies and Tools to Engineer Plant Pathways
Metabolic Engineering for Production of Terpenoids
Pharmaceutical Terpenoids
Industrially Important Terpenes
Limitations and Challenges
Conclusion: A Shift Towards a Biobased Economy
References
Chapter 19: Ethical Perspectives and Limitations of Metabolic Engineering Technologies in Plants
Introduction
Limitations and Ethical Perspectives of Metabolic Engineering
Conclusion and Future Perspectives
References
π SIMILAR VOLUMES
Written by leading international experts in the field of plant metabolic engineering, this book discusses, mainly, applications of plant metabolic engineering. Metabolic engineering, which is part of second generation biotechnology, has made several contributions at basic level as well as at concept
<p><P>Written by leading international experts in the field of plant metabolic engineering, this book discusses, mainly, applications of plant metabolic engineering. Metabolic engineering, which is part of second generation biotechnology, has made several contributions at basic level as well as at c
<p><i>Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B</i>, the latest volume in the <i>Methods in Enzymology </i>series,<i> </i>continues the legacy of this premier serial with quality chapters authored by leaders in the field. </p> <p>This volume covers research methods,
<p><span>Plants have evolved an amazing array of metabolic pathways leading to molecules capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals,