Metabolic effects of acetaminophen. Studies in the isolated perfused rat liver

✍ Scribed by Ana Maria Itinose; Marlene Leiko Doi Sakuno; Adelar Bracht

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 989 KB
• Volume: 7
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290070405

✦ Synopsis

The effects of acetaminophen on the metabolism of the isolated perfused rat liver were investigated. The following results were obtained:

(1) Acetaminophen increased glucose release and glycolysis from endogenous glycogen (glycogenolysis).

(2) Oxygen uptake, gluconeogenesis from either pyruvate or fructose and glycogen synthesis were inhibited.

(3) In isolated rat liver mitochondria acetaminophen decreased state I11 and state 1V respiration; it also (4) The action of acetaminophen on glycogenolysis was not affected by N-acetylcysteine; this compound, ( 5 ) The effects of acetaminophen are reversible. decreased the ADP/O ratio and the respiratory control ratio. however, increased glycogen synthesis.

It was concluded that glycogen depletion by acetaminophen can be produced by two mechanisms. The first, as previously demonstrated by several workers, depends on irreversible binding of a reactive metabolite. The second, however, is reversible and depends primarily on an inhibition of mitochondria1 energy metabolism.