Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells
✍ Scribed by Anna E. Hills; Ashvin Patel; Paul Boyd; David C. James
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 246 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0006-3592
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We previously compared changes in individual protein abundance between the proteomes of GS-NS0 cell lines with varying rates of cell-specific recombinant monoclonal antibody production (qMab). Here we extend analyses of our proteomic dataset to statistically determine if particular cell lines have d
An amplified NS0 cell line transfected with a vector expressing a humanized monoclonal antibody (MAb) against CD-18 and glutamine synthetase (GS) was cultivated in a 1.5 L fed-batch culture using a serum-free, glutamine-free medium. Concentrated solutions of key nutrient components were fed periodic
## Abstract Presented is a novel antibody production platform based on the fed‐batch culture of recombinant, NS0‐derived cell lines. A standardized fed‐batch cell culture process was developed for five non‐GS NS0 cell lines using enriched and optimized protein‐free, cholesterol‐free, and chemically
## Abstract Presented is an antibody production platform based on the fed‐batch culture of recombinant NS0‐derived cell lines. NS0 host cells, obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK, Part No. 85110503), were first adapted to grow in a protein‐free, cholesterol‐