Metabolic Changes Associated with Vacuolation in Murine Models of Scrapie using In Vitro1H-NMR Spectroscopy
✍ Scribed by Yuen-Li Chung; Alun Williams; Angela Chong; James Hope; Steve C. R. Willims; Jimmy D. Bell
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 469 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0952-3480
No coin nor oath required. For personal study only.
✦ Synopsis
In this study, metabolic changes in the 79A/C3H, ME7NM, ME7/C3H, 87VNM and 22NSV scrapie mouse models were investigated during the clinical phase of the disease, using in vitro proton nuclear magnetic resonance spectroscopy. N-acetyl-aspartate was found to be significantly reduced in infected mice of the ME7/ C3H (40% reduction), ME7NM (26%) and 79A/C3H (17%) models when compared to control mice, but not in the 87VNM and 22A/SV models. The concentration of choline containing compounds and creatine remain unchanged in all models when compared with control murine brains. The level of N-acetyl-aspartate reduction correlated with the extent of grey-matter brain vacuolation. The levels of myo-inositol were found to be significantly increased in the ME7NM (143%) and 79A/C3H (132%) models only and no significant changes were observed in the ME7/C3H, 87VNM and 22A/SV models. These changes did not correspond to the severity of gliosis. 0 1997 by John Wiley & Sons, Ltd.