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Metabolic Breakdown of [3H]thymidine and the Inability to Measure Human Lymphocyte Proliferation by Incorporation of Radioactivity

โœ Scribed by Judy Bodycote and Sheldon Wolff


Book ID
123632905
Publisher
National Academy of Sciences
Year
1986
Tongue
English
Weight
922 KB
Volume
83
Category
Article
ISSN
0027-8424

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Proliferation of human lymphocytes in response to various stimuli has traditionally been assessed by measuring uptake of radiolabeled nucleotides such as3H-thymidine. We have evaluated a fluorometric assay, which uses the commercially available reagent, alamarBlue, as a potential substitute for the3