Meta-Iodobenzylguanidine (mIBG) uptake and storage in the human neuroblastoma cell line SK-N-BE(2C)

✍ Scribed by L. S. Lashford; J. P. Hancock; J. T. Kemshead

• Book ID: 102866454
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 590 KB
• Volume: 47
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910470119

✦ Synopsis

Whilst many human neuroblastorna cell lines have been studied to see if they are capable of taking up mlBG, few appear to have this ability. This contrasts markedly to the situation in vivo, where uptake has been demonstrated in the majority of tumours investigated. Here we report on the human neuroblastoma cell line SK-N-BE(2C) and demonstrate that mlBG uptake can occur in this cell line through 2 mech-

anisms. At low concentrations of mlBG (approximately lo-"

M) an active transport process predominates, whereas at nonphysiological levels (I 0-' M) uptake occurs through passive diffusion. The active transport process is ATP-, Na+-and ternperature-dependent. Uptake is blocked by n desipramine, an inhibitor of the uptake-I mechanism involved in amine transport. In contrast, desipramine has no effect on the passive diffusion of mlBG into cells. The active transport mechanism for mlBG uptake appears rather promiscuous for biogenic amines, as dopamine, tyramine and nor-adrenaline were highly efficient at blocking mlBG entry to the cell. Serotonin and histamine were capable of interfering with mlBG uptake only at much higher concentrations. Electron microscopy of SK-N-BE(2C) cells revealed a paucity of neurosecretory granules. Biochemical investigations demonstrated the majority of mlBG to be present in the cytoplasm of cells. The availability of a human neuroblastoma cell line that grows well, both as xenograft and in culture, should further our understanding of the cytotoxic effects of mlBG and thus enhance its clinical usefulness.