Messung des H�moglobingehalts von frischem Blut durch atomabsorptionsspektralphotometrische Eisenbestimmung
✍ Scribed by L�tterle, J.
- Book ID
- 104661011
- Publisher
- Springer-Verlag
- Year
- 1980
- Tongue
- English
- Weight
- 373 KB
- Volume
- 85
- Category
- Article
- ISSN
- 1437-1596
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✦ Synopsis
Measurement of the Hemoglobin Content of Fresh Blood by Atomic-absorption-spectrophotometric Iron Determination
Summary. Description of an atomic-absorption-spectrophotometric iron de- termination in whole blood for the measurement of hemoglobin content. Its use is recommended for Hb determination in decayed blood because in such cases the photometric Cyan-Met-Hb method is not always reliable. To 5 ml aqueous Triton-X-100 solution (0.5%) 0.1ml blood are added. Measuring is done after hemolysis has occurred. The calibration of the atomic-absorption spectrophotometer is performed with a standard of 10gg Fe/ml H20. Samples with a Fe-content from 1 gg to 20 ~tg/ml H20 (1.6 g-32 g Hb/100 ml) are in the linear range of the apparatus. There is a linear correlation between the results of this and the photometric method.