Environmentally induced mutations, especially those involving large scale genetic damage such as deletions and chromosome loss, are of central importance in the production of human genetic disease and cancer. We have developed a methodology, the AL assay, that permits detection of such extensive gen
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Messenger RNA quantification after fluorescence-activated cell sorting using in situ hybridization
โ Scribed by Hiroya Yamada; Rie Maruo; Mikio Watanabe; Yoh Hidaka; Yoshinori Iwatani; Toru Takano
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 226 KB
- Volume
- 77A
- Category
- Article
- ISSN
- 0196-4763
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We studied the feasibility and the sensitivity of fluorescent in situ hybridization (FISH) using leukemic or host/donor-specific probes on flow-sorted cells to assess minimal residual disease (MRD) or chimerism in transplanted patients in complete remission. We first performed experimental models of