Messenger RNA for the insect storage protein calliphorin:In vitrotranslation and chromosomal hybridization analyses of a 20 S poly(A)-RNA fraction
✍ Scribed by D. J. Kemp; J. A. Thomson; W. J. Peacock; T. J. V. Higgins
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 854 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-2928
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✦ Synopsis
A major poly(A)-containing RNA fraction of the approximate size expected of a monocistronic mRNA for the storage protein calliphorin has been isolated from the larval fat bodies of Calliphora vicina during early instar 3. This 20 S RNA fraction programs the synthesis by cell-free wheat embryo extracts of polypeptides of 86,000 daltons identified by tryptic peptide fingerprinting as precursors of the authentic calliphorin subunits of 83,000 daltons. Complementary DNA synthesized by AMV reverse transcriptase using the same 20 S RNA as template hybridized in situ to a single segment of one or two bands in the salivary polytene chromosomes of C. vicina.