Messenger RNA doing without poly(A)
β Scribed by LEWIN, BENJAMIN
- Book ID
- 109693469
- Publisher
- Nature Publishing Group
- Year
- 1974
- Tongue
- English
- Weight
- 264 KB
- Volume
- 252
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/252268b0
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π SIMILAR VOLUMES
A 9S RNA fraction from mouse reticulocytes, containing the active Ξ±- and Ξ²-globin mRNAs, has been isolated by hybridization of the polyadenylate regions in the mRNAs to oligo(dT)-cellulose. The adenylate-rich sequence isolated by limited RNase digestion of the globin mRNAs migrates between 4S and 5S
Cell suspension cultures from parsley (Petroselinum hortense Hoffm.) were labelled in vivo with [2 -3 H] adenosine. The RNA isolated from the ribosomal pellet was fractionated on an oligo(dT)-cellulose column. Approximately 1.5% of the RNA, representing about 15% of the total radioactivity, was reta