Mesothelioma in rats following intrapleural injection of chrysotile and phosphorylated chrysotile (chrysophosphate)
✍ Scribed by A. Van Der Meeren; J. Fleury; M. Nebut; G. Monchaux; X. Janson; M. C. Jaurand
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- French
- Weight
- 708 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Pathological effects of asbestos are probably dependent on the size and surface properties of the fibers. Surface-modified chrysotile fibers were injected into the pleural cavity of rats to investigate the potency of the fiber to induce mesothelioma. Chrysotile fibers were modified by a phosphorylation process, resulting in the presence of phosphorus at the fiber surface. Phosphorylated samples were characterized by enhanced durability and reduced affinity for biological macromolecules. Five samples were tested: I untreated and 4 phosphorylated. ChrP I, ChrP2 and ChrP3 corresponded to phosphorylated samples obtained by first, second and third passages through an Alpine classifier; Pm was defibrillated ChrP I. The number of fibers per microgram and the size distribution were determined by transmission electron microscopy and classified in 4 size groups.
Groups of 35 rats were inoculated with 20 mg of fibers suspended in 0.9% NaCl solution. No mesothelioma was found in the saline controls. All fiber samples were proficient in producing mesothelioma; the percentages were different between groups and untreated chrysotile but not significantly so. The differences may be explained on the basis of the number of fibers injected which were > 8 pm in length and < 0.25 prn in diameter. The findings of a proficiency of long fibers to produce mesothelioma, previously reported by others for glass fibers, could be applied to chrysotile.