Membrane region of surface IgM is not sufficient for transducing growth inhibitory signals in an immature B cell line WEHI-231
✍ Scribed by Naoto Iwabuchi; Douglas B. Williams; Hai P. Nguyen; Yongjian Wu; Roland Tisch; Toshifumi Azuma; M. James Phillips; Nobumichi Hozumi
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 527 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Membrane region of surface IgM is not sufficient for transducing growth inhibitory signals in an immature B cell line WEHI-231"
The murine B lymphoma line WEHI-231 is representative of immature B cells. Like normal immature B cells,WEHI-231 is susceptible to growth arrest following cross-linking of surface IgM (sIgM). Previously, we have shown using a WEHI-231 immunoglobulin (Ig) 6-transfectant that sIgD cross-linking failed to initiate growth arrest, in contrast to sIgM. In this report, we extend our research to investigate the structural requirement of Ig y chain for regulating growth inhibition. Recombinant, chimeric Ig molecules 6/y, and p/8, consisting of exons encoding extracellular 6 and p domains and membrane regions of different isotypes were constructed and introduced into WEHI-231 cells. A similar approach was used for sIgG2b-expressing transfectants. Our findings indicate that the p , region is not sufficient for regulation of growth inhibition in WEHI-231 cells and suggest that additional extracellular region(s) of p chain may be required for this response.