Membrane Receptors: Methods for Purification and Characterization

✍ Scribed by Morley D. Hollenberg, Ebba Nexø (auth.), S. Jacobs, P. Cuatrecasas (eds.)

• Publisher: Springer Netherlands
• Year: 1981
• Tongue: English
• Leaves: 246
• Series: Receptors and Recognition
• Edition: 1
• Category: Library

✦ Synopsis

Hardly a decade ago, membrane receptors were an attractive but largely unproven concept. Since that time enormous progress has been made, and we are now able to consider receptors much more concretely. Their existence has been established, their binding properties have been determined, and in some cases, they have been highly purified and their physical-chemical properties studied. It is now even possible to visualize microscopically some receptors. This progress has resulted largely from the development of highly powerful methods. These methods are the subject of this volume. Although considerably diverse, different receptors share certain common pro­ perties, and common problems are encountered in their study. Consequently, a small number of techniques are particularly useful in studying different types of receptors. Thus, it makes sense to speak about membrane receptor methodology. A very apparent problem in the study of membrane receptors is their presence in exceedingly small quantities and in a highly impure state. Therefore, very sensitive and specific techniques are required for their detection, characterization and purification. Such sensitivity and specificity is provided by the ability of receptors to bind certain ligands with very high affmity, and it is not surprising that most of the methods described in this volume depend upon this high affinity binding. The antigen-antibody interaction is of comparable sensitivity and speci­ ficity. Recently, a number of anti-receptor antibodies have been produced or found to occur spontaneously in auto-immune diseases. Undoubtedly, more will be produced in the future.

✦ Table of Contents

Front Matter....Pages i-xii
Receptor Binding Assays....Pages 1-31
Solubilization and Characterization of Membrane Proteins....Pages 33-59
Affinity Chromatography for Membrane Receptor Purification....Pages 61-86
Affinity Labeling of Hormone Receptors and Other Ligand Binding Proteins....Pages 87-113
Immunochemical Identification and Characterization of Membrane Receptors....Pages 115-156
Quantitative Methods for Studying the Mobility and Distribution of Receptors on Viable Cells....Pages 157-170
Somatic Genetic Analysis of Hormone Action....Pages 171-201
Some Perspectives on the Hormone-stimulated Adenylate Cyclase System....Pages 203-236
Back Matter....Pages 237-240

✦ Subjects

Science, general