The conditions for obtaining highly concentrated salt-free protein solutions have been examined. It was found that a convenient and rapid method consists of combining ion-exchange and gel-filtration chromatography in one column. We observed that a volume ratio of Sephadex G-25 to ion exchanger of 2.
β¦ LIBER β¦
Membrane Proteins and Impure Detergents: Procedures to Purify Membrane Proteins to a Degree Suitable for Tryptophan Fluorescence Spectroscopy
β Scribed by Dolf Swaving Dijkstra; Jaap Broos; George T. Robillard
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 135 KB
- Volume
- 240
- Category
- Article
- ISSN
- 0003-2697
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