Membrane protein structure and function characterization : methods and protocols

✍ Scribed by Lacapère, Jean-Jacques

• Publisher: Humana Press
• Year: 2017
• Tongue: English
• Leaves: 421
• Series: Methods in molecular biology 1635
• Category: Library

✦ Synopsis

In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field. . Read more...

Abstract:

In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Read more...

✦ Table of Contents

Front Matter ....Pages i-xii
Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2 (Lucile Senicourt, Soria Iatmanen-Harbi, Claude Hattab, Mariano Anibal Ostuni, Marie-France Giraud, Jean-Jacques Lacapere)....Pages 1-25
Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex (Hajer Souabni, Aymen Ezzine, Tania Bizouarn, Laura Baciou)....Pages 27-43
Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts (Lucie Hartmann, Estelle Metzger, Noémie Ottelard, Renaud Wagner)....Pages 45-56
Cell-Free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-Synthase Subunits (Isabelle Larrieu, James Tolchard, Corinne Sanchez, Edmond Yazo Kone, Alexandre Barras, Claire Stines-Chaumeil et al.)....Pages 57-90
Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins (Marie-Laure Fogeron, Aurélie Badillo, François Penin, Anja Böckmann)....Pages 91-108
Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins (Vilius Kurauskas, Paul Schanda, Remy Sounier)....Pages 109-123
Labeling of Membrane Complexes for Electron Microscopy (Francesca Gubellini, Rémi Fronzes)....Pages 125-138
Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor (Ghérici Hassaïne, Cédric Deluz, Luigino Grasso, Romain Wyss, Ruud Hovius, Henning Stahlberg et al.)....Pages 139-168
Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization (Françoise Bonneté, Patrick J. Loll)....Pages 169-193
Secondary Structure Determination by Means of ATR-FTIR Spectroscopy (Batoul Srour, Stefan Bruechert, Susana L. A. Andrade, Petra Hellwig)....Pages 195-203
Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins (Jeroen F. van Dyck, Albert Konijnenberg, Frank Sobott)....Pages 205-232
Mass Spectrometry of Mitochondrial Membrane Protein Complexes (Luc Negroni, Michel Zivy, Claire Lemaire)....Pages 233-246
Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+ NQR from V. cholerae in the Presence of Lipids (Yashvin Neehaul, Sebastien Kriegel, Blanca Barquera, Petra Hellwig)....Pages 247-257
Reconstitution of Membrane Proteins in Liposomes (Alice Verchère, Isabelle Broutin, Martin Picard)....Pages 259-282
Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes (Michel Vivaudou, Zlatomir Todorov, Gina Catalina Reyes-Mejia, Christophe Moreau)....Pages 283-301
The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein (Philippe Rosier, Frédéric Gélébart, Nicolas Dumesnil, Gauthier Esnot, Manuela Dezi, Marc Morand et al.)....Pages 303-316
Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy (Laetitia Daury, Jean-Christophe Taveau, Dimitri Salvador, Marie Glavier, Olivier Lambert)....Pages 317-327
Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO (Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos, Jean-Jacques Lacapere)....Pages 329-344
Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR (Denis Lacabanne, Britta Kunert, Carole Gardiennet, Beat H. Meier, Anja Bo¨ckmann)....Pages 345-358
Simulation of Ligand Binding to Membrane Proteins (Samuel Murail)....Pages 359-381
Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO (Aurore Vaitinadapoule, Catherine Etchebest)....Pages 383-416
Back Matter ....Pages 417-419

✦ Subjects

Membrane proteins -- Laboratory manuals;Membrane Proteins;Membrane proteins