Membrane Protein Protocols
โ Selinsky
๐ Library
๐
2010
๐ Humana Press
๐ English
โฆ LIBER โฆ
๐
Membrane Protein Protocols
โ Scribed by Barry S. Selinsky
- Publisher
- Humana Press
- Year
- 2010
- Tongue
- English
- Leaves
- 307
- Series
- Methods in Molecular Biology
- Edition
- 1st Edition.
- Category
- Library
โฌ Acquire This Volume
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โฆ Synopsis
A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
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<p>Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at ma