✍ Scribed by Carla M. Franzin; Peter Teriete; Francesca M. Marassi
No coin nor oath required. For personal study only.
Corticosteroid hormone-induced factor (CHIF) is a major regulatory subunit of the Na,K-ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid-state NMR orientation-dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid-state NMR spectrum measured for (15)N-labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane.
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## Abstract The complete assignment of cholesterol ^1^H and ^13^C NMR resonances in a lipid bilayer environment (L~α~‐dimyristoylphosphatidylcholine/cholesterol 2:1) has been obtained by a combination of 1D and 2D MAS NMR experiments: ^13^C spectral editing, ge‐HSQC, dipolar HETCOR and J‐based HETC