Changes in the intracellular Ca2+ concentration ([Ca2+]i) and the formation of actin filaments were investigated in unfertilized eggs of the sea urchin Hemicentrotus pulcherrimus after activation with a phorbol ester, 12-O-tetradecanoyl phorbol13-acetate (TPA). Intracellular Ca2+ oscillation was obs
✦ LIBER ✦
Membrane formation and cleavage in unilaterally irradiated sea urchin eggs
✍ Scribed by Spikes, John D.
- Publisher
- John Wiley and Sons
- Year
- 1944
- Tongue
- English
- Weight
- 822 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0022-104X
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## Abstract We demonstrate that complete sea urchin male pronuclear development in vitro is a two‐step process involving membrane‐independent chromatin decondensation and nuclear envelope‐dependent pronuclear swelling. In the absence of cytoplasmic membrane vesicles (MVs), permeabilized sperm chrom