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Membrane chromatography of different molecular weight polyethylene glycols on polyvinylchloride ultrafilters
In a previous communication1 we described the group separation of high-and low-molecular-weight polyethylene glycols (PEG) by membrane chromatography on cellulose nitrate. The behaviour of the PEG was generally similar to that of proteins and amino acids on the same carriersa. Quite recently, we have reported314 that polyvinylchloride (PVC) membrane filters were suitable for use as chromatographic carriers and that they adsorbed proteins more firmly and over a much broader range of pH and molecular weights than nitrocelluloseG. Furthermore, we noted that PEG 20 ooo was also firmly adsorbed to PVC in a similar way.
In the present paper, we have investigated whether a quantitative microestimation of PEG could be achieved by membrane chromatography on PVC filters in a similar manner to that observed with proteins3s4, and if so, whether there is any noticeable relationship between the molecular weights of different PEG fractions and the areas of the corresponding PEG layers. The last question was intended as a simple model for analoguous experiments with proteins.
Materials and methods
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