Melting temperatures of the cohesive ends of some non-inducible coliphage DNA's
β Scribed by Mandel, M.; Berg, A.
- Book ID
- 123469010
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 192 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0022-2836
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The half molecules of 186 DNA have been isolated by the Hg(II)-CszSOa density gradient centrifugation technique. The buoyant densities of the two halves in CsCl at 25Β°C. are 1.713 and 1.709 g . / ~m . ~, corresponding to GC contents of 54% and 50%, respectively. Similarly, 5-bromouracil labeled X DN
## Abstract This report presents a quantitative test of the ability of the counterion condensation theory to describe the protonβinduced lowering of DNA melting temperature. From a general approach of Record et al. [Record, M. T., Anderson, C. F. & Lohman, T. H. (1978) __Q. Rev. Biophys.__ **11**,