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Melting of DNA in ethanol–water solutions

✍ Scribed by A. F. Usatyi; L. S. Shlyakhtenko

Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
545 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The thermal denaturation curves of DNA have been investigated over a wide range of temperatures T and ethanol concentrations C by CD and uv absorption methods. The phase diagram of conformational DNA states has been constructed in T,C plane. The range of the A, B, and coiled forms of DNA was determined. The behavior of the DNA denaturation curve in the neighborhood of the triple point shows that the conformational transition B → A is realized by short parts of the DNA double helix. This is the reason for the coincidence of the melting temperatures of the A and B forms of DNA throughout the range of their coexistence.

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