The equilibrium shapes of supercoiled DNA are investigated by employing an elastic model. First, a set of Euler equations is derived to determine the equilibrium shapes under ring-closure conditions. Two exact solutions that describe circular and figure-8 shapes are obtained. Using these and their t
Melting characteristics of highly supercoiled DNA
β Scribed by Srikanta Sen; Ansuman Lahiri; Rabi Majumdar
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 481 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0301-4622
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π SIMILAR VOLUMES
The melting curve for a covalently closed circular DNA has been analyzed on the hasis of an expreasion for the supercoiling energy derived in terms of the elastic parameters of the macromolecule, treated as a homopolymer. The result obtained by applying the usual methods of statistical mechanics ind
A flow linear dichroism technique is utilized to study the unwinding of supercoiled DNA induced by the binding of ethidium bromide ( E B ) and proflavine ( PF) at different ratios r (drug added/DNA base). In the case of either EB or PF bound to linear calf thymus DNA, the reduced linear dichroism si
## Abstract Covalently closed circular DNA can exist in different configurations known as __circular__, __toroidal__, and __interwound__. Changes among these forms can be made in several ways, including the insertion of dye molecules between adjacent base pairs, which tends to untwist the doubleβhe