Melanoma cell lysis by human CTL clones: Differential involvement of T3, T8 and HLA antigens

✍ Scribed by Giuseppe Fossati; Andrea Anichini; Giorgio Parmiani

• Book ID: 102863942
• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: French
• Weight: 607 KB
• Volume: 39
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910390606

✦ Synopsis

Three lymphocyte clones, derived by micromanipulation from peripheral blood lymphocytes (PBL) of a melanoma patient and expressing a broad pattern of reactivity against different target cells, were analyzed for the involvement of T-cell markers and HLA antigens in the lysis of target cells by blocking experiments with a panel of monoclonal antibodies (MAbs). The clones lysed autologous melanoma cells (Me 28) and 18 out of 22 allogeneic targets including neoplastic and normal cells of different histological origin. Anti-13 and anti-1 8 MAbs strongly inhibited the cytotoxicity of the lymphocyte clones against Me 28, 3 allogeneic melanomas and 3 carcinomas, but failed to affect the lysis of K562. Anti-HLA class-I MAb (w6/32) produced a significant enhancement of the iysis of Me 28 by the 3 clones without modifying cytotoxicity against one allogeneic melanoma or against K562 cells. Anti-HLA class-ll MAb (DI.12) did not affect the lysis of the same targets by the 3 clones. These results thus indicate that some anti-melanoma CTL clones may interact with autologous tumor cells by the T3 and T8 structures in an HLA class-I unrestricted manner.