Melanoma antigen-encoding gene-1 expression in invasive gastric carcinoma: Correlation with stage of disease

✍ Scribed by Katano, Mitsuo; Nakamura, Mitsunari; Morisaki, Takashi; Fujimoto, Kazuma

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 156 KB
• Volume: 64
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/(sici)1096-9098(199703)64:3<195::aid-jso4>3.0.co;2-5

✦ Synopsis

Background:

A human melanoma antigen-encoding gene-1, mage-1 gene, may be linked to the neoplastic transformation. in the present study, we extended this association with human gastric carcinomas. specifically, we focused on the relationship between mage-1 gene expression and the histologic stage of gastric carcinoma.

Methods:

We used a reverse transcription-polymerase chain reaction assay (rt-pcr) to analyze the expression of the mage-1 gene in 38 endoscopic biopsy specimens from gastric carcinomas. we also studied the relationship between the expression of mage-1 gene and the genetic expression of several tumor invasion-related factors, including 72 kd type iv collagenase (mmp2), urokinase-type plasminogen activator (upa), platelet-derived growth factor a (pdgf-a), and vascular endothelial growth factor (vegf).

Results:

Eleven of the 38 tumor samples (28.9%) expressed the mage-1 gene. mage-1 gene expression was present only in two of the 38 adjacent nontumor samples (5.3%). mage-1 gene expression in the 38 tumor samples was significantly correlated with the histological stage of disease (p = 0.0008), especially with the depth of histologically confirmed tumor invasion (t1 vs. t2 or greater, p = 0.00048). the expression of mage-1 gene correlated with the expression of mmp2 (p = 0.0064), upa (p = 0.0390), and pdgf-a (p = 0.00018).

Conclusions:

These data suggest that the mage-1 gene may be activated in gastric carcinomas during periods of their development or invasion. in addition, a relationship between mage-1 gene expression and expression of invasion-related factors has been demonstrated.