Meiotic doubling of chromosome number in Festulolium

✍ Scribed by J. Dijkstra; A. L. F. Vos

• Publisher: Springer
• Year: 1975
• Tongue: English
• Weight: 485 KB
• Volume: 24
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00132913

✦ Synopsis

Meiotic doubling of the chromosome number of Festulolium hybrids was applied. Isolated, male-sterile tetraploid LpFa3 hybrids from the cross diploid Lolium perenne x hexaploid Festuca arundinacea, were open-pollinated by octoploid L,Fao's. The percentage successful doubling (percentage of flowering tetraploid plants, which gave seedlings) x (percentage of these seedlings with the doubled number of chromosomes) was for 157 clones, in 1972, 45°;; for 407 clones in 1974 it was considerably smaller, i.e. 7? 0.

Data of our own experiments and experiments from the literature with meiotic and mitotic doubling of the chromosome number of LFa3 hybrids were compared. The percentage of successful meiotic doubling for three experiments was, on average, 38u£,. For germinated seeds and tillers mitotic doubling by colchicine treatment yielded, on average, 11 o£ (three experiments) and 3~°Jo (four experiments), respectively.

In addition to the fact that meiotic doubling, on average, gives better results than mitotic doubling, there are the advantage of an absence of mixoploid plants and the advantage of a perhaps greater heterozygosity. It is likely that application of meiotic doubling facilitates the production of fertile Festulolium hybrids and of other intergeneric or interspecific fertile hybrids in which meiotic doubling is possible.