𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Megakaryocyte colony growth-supporting activities in human plasma: Modification by platelets and platelet membranes

✍ Scribed by Kazuo Yamasaki; Nazir Jamal; Kenneth G. Mann; Hans A. Messner

Book ID
102885011
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
713 KB
Volume
133
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Human megakaryocyte colonies are grown in methylcellulose with plateletpoor plasma and medium conditioned by phytohemagglutinin-stimulated leukocytes (PHA-LCM) as a source of megakaryocyte colony stimulating factor (MEG-CSF). The megakaryocyte colony growth-supporting activity in human plasma can be absorbed by intact platelets or degranulated platelet membranes. It was possible to recover the activity by solubilizing platelet mem- branes with cholic acid. Filtration of the solubilized platelet membrane preparations through a Sephadex GI00 column yielded at least two activity peaks. The molecular weight of these two activities differs from that of the growth-promoting activity in PHA-LCM.

A variety of culture conditions facilitate the growth of megakaryocyte colonies from human bone marrow and peripheral blood . The essential components for megakaryocyte colony formation in methylcellulose include human plasma and a source of megakaryocyte colony stimulating factor (MEG-CSF) . The latter may be provided by phytohemagglutinin-stimulated leukocyte conditioned medium (PHA-LCM), or more recently by recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) (

📜 SIMILAR VOLUMES

Enhancement of vascularization and granu
Enhancement of vascularization and granulation tissue formation by growth factors in human platelet-rich plasma-containing fragmin/protamine microparticles
✍ Megumi Takikawa; Shin-Ichiro Nakamura; Shingo Nakamura; Masaki Nambu; Masayuki I 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 442 KB

## Abstract The purpose of this study was to evaluate effects of human platelet‐rich plasma (PRP)‐containing fragmin/protamine microparticles (F/P MPs) as a protein carrier on neovascularization and granulation tissue formation. Frozen and thawed PRP contains high concentrations of various growth f

Conditions influencing release of granul
Conditions influencing release of granule contents from human platelets in citrated plasma induced by ADP or the thrombin receptor activating peptide SFLLRN: Direct measurement of percent release of β-thromboglobulin and assessment by flow cytometry of P-selectin expression
✍ Rand, Margaret L.; Perry, Dennis W.; Packham, Marian A.; Gemmell, Cynthia H.; Ye 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 695 KB

Contrary to a recent report [Rinder et al.: Blood 82:505, 19931, aspirin does inhibit the release of a-granule contents as well as inhibiting the release of dense granule contents by human platelets during ADP-induced aggregation in citrated platelet-rich plasma (PRP). Measurements were: percent rel