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Megabase methods: A quantum jump in recombinant DNA techniques

✍ Scribed by Bertrand R. Jordan

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
794 KB
Volume
8
Category
Article
ISSN
0265-9247

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✦ Synopsis

Until quite recently, recombinant DNA technology was not able to deal with DNA molecules larger than 20-40 kb. This is a serious limitation for the study of mammalian, and in particular human genomes whose total length is approx. 3 x 106 kb, since the best resolution of genetic and chromosomal analysis is usually the rough equivalent of 1000-5000 kb. Three recently developed methods promise to bridge this gap: pulsed field gel electrophoresis, which can analyze megabase-sized DNA fragments; cloning in yeast, which can clone and propagate DNA fragments of several hundred kb; and jumping libraries, which allow 'jumping' over large distances along the chromosome. This review presents the current status of these very promising technologies.

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