One of the key steps of structural genomics and proteomics is high-throughput expression of many target proteins. Gene cloning, especially by ligation-independent cloning techniques, and recombinant protein expression using microbial hosts such as Escherichia coli and the yeast Pichia pastoris are w
โฆ LIBER โฆ
Medium-Scale Structural Genomics: Strategies for Protein Expression and Crystallization
โ Scribed by Renaud Vincentelli; Christophe Bignon; Arnaud Gruez; Stephane Canaan; Gerlind Sulzenbacher; Mariella Tegoni; Valerie Campanacci; Christian Cambillau
- Publisher
- John Wiley and Sons
- Year
- 2003
- Weight
- 57 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0931-7597
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Protein expression systems for structura
โ
Shigeyuki Yokoyama
๐
Article
๐
2003
๐
Elsevier Science
๐
English
โ 98 KB
A flexible and economical medium-through
โ
Moreland, Nicole ;Ashton, Rachael ;Baker, Heather M. ;Ivanovic, Ivan ;Patterson,
๐
Article
๐
2005
๐
International Union of Crystallography
๐
English
โ 967 KB
Large-scale structural genomics centres rely heavily on robotics to ensure that maximum throughput is achieved. However, the size and cost of these approaches is out of the reach of most academic structural biology efforts. A major challenge for such groups is to adapt current high-throughput scheme