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Mediation of glucocorticoid receptor function by the activation of latent transforming growth factor beta 1 in MG-63 human osteosarcoma cells

✍ Scribed by Jocelyn Boulanger; Carlos Reyes-Moreno; Michael Koutsilieris

Publisher
John Wiley and Sons
Year
1995
Tongue
French
Weight
912 KB
Volume
61
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

We analyzed glucocortcoid receptor function using ligand binding assays, DNA band‐shift analysis and trans‐activation of the murine mammary tumor virus‐thymidine kinase‐chloramphenicol acetyltransferase reporter gene in transiently transfected MG‐63 human osteosarcoma cells. Dexamethasone increased the distribution of MG‐63 cells in the G~1~/G~0~ phase of the cell cycle, thus decreasing the rate of DNA synthesis and cell growth. Its effect on MG‐63 cell growth was neutralized by RU486 and anti‐transforming growth factor beta 1 (TGFβ1) antibody. In addition, (i) dexamethasone increased the levels of active TGFβ1 in MG‐63‐conditioned media without significantly altering the expression of TGFβ1 mRNA in MG‐63 cells and (ii) TGFβ1 inhibited proliferation of MG‐63 cells. Therefore, we conclude that glucocorticoid receptor function is mediated by the activation of latent‐TGFβ1 in MG‐63 osteosarcoma cells. © 1995 Wiley‐Liss, Inc.

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