Media suitable for industrial production of Entomophthora virulenta zygospores

✍ Scribed by J.-P. Latgé; R. S. Soper; C. D. Madore

• Book ID: 102764209
• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 699 KB
• Volume: 19
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260190903

✦ Synopsis

Abstract

Entomophthora virulenta has been grown using inexpensive, commercial grade nutrient sources in order to produce a resting spore formulation available to agriculture. Solid media and liquid media in shake flasks and fermentors were examined. The best carbon sources for sporulation were dextrose and corn syrup, while the best nitrogen sources were yeast extract, soybean, and cottonseed flours. The optimal nutrient concentration (12%) produced 3 × 10^6^spores/ml with a 70% germination rate within five days.