Mechanized systems for media dispensing, inoculation, and replication of microorganisms

Kluyveromyces lactiskiller system:ORF1of

Kluyveromyces lactiskiller system:ORF1of pGKL2 has no function in immunity expression and is dispensable for killer plasmid replication and maintenance

✍ R. Schaffrath; M. J. R. Stark; N. Gunge; F. Meinhardt 📂 Article 📅 1992 🏛 Springer-Verlag 🌐 English ⚖ 929 KB

To functionally characterize the genes encoded by the larger killer plasmid pGKL2 from Kluyveromyces lactis a previously developed in-vivo recombination system was exploited. An in-vitro modified version of the cytoplasmically expressible LEU2 gene cartridge (LEU2\*) flanked by appropriate pGKL2 seg