Mechanistic Roles of Tyrosine 149 and Serine 124 in UDP-galactose 4-Epimerase from Escherichia coli †
✍ Scribed by Liu, Yijeng; Thoden, James B.; Kim, Jeongmin; Berger, Elizabeth; Gulick, Andrew M.; Ruzicka, Frank J.; Holden, Hazel M.; Frey, Perry A.
- Book ID
- 126828575
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 312 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0006-2960
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Uridine diphosphogalactose-4-epimerase from E. coli has been crystallized in a form suitable for a high-resolution X-ray crystallographic structural analysis. The enzyme complexed with a substrate analogue, uridine diphosphobenzene (UDP-benzene), crystallizes readily using polyethylene glycol 8000 a
## Abstract UDP‐galactose 4‐epimerase catalyzes the conversion of UDP‐galactose to UDP‐glucose during normal galactose metabolism. The molecular structure of UDP‐galactose 4‐epimerase from __Escherichia coli__ has now been solved to a nominal resolution of 2.5 Å. As isolated from __E. coli__, the m