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Mechanisms responsible for the limited lifespan and immortal phenotypes in cultured mammalian cells

✍ Scribed by R.F. Rosenberger; E. Gounaris; E. Kolettas


Book ID
108359909
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
673 KB
Volume
148
Category
Article
ISSN
0022-5193

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## Abstract There are a growing number of reports on the sub‐physiological temperature culturing (<37Β°C) of mammalian cells for increased recombinant protein yield, although the effect is variable between cell lines, expression systems, and the product of interest. What is becoming clear is that ex