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Mechanisms of resistance to Leishmania aethiopica. I. Interferon-γ in combination with a cytokine (not tumor necrosis factor-α) is required, but cannot act alone in the inhibition of intracellular forms of L. aethiopica in THP1 cells

✍ Scribed by Abdi Muse Mohamed; Tesema Taye; Hannah O. Akuffo


Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
776 KB
Volume
22
Category
Article
ISSN
0014-2980

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✦ Synopsis


Mechanisms of resistance to Leishmania aethiopica. I. Interferon-y in combination with a cytokine (not tumor necrosis factor-a) is required, but cannot act alone in the inhibition of intracellular forms of L. aethiopica in THPl cells* Following exposure to promastigotes of various Leishmania species, mononuclear cells from non-exposed as well as potentially exposed individuals produced a cytokine response which inhibited intracellular forms of Leishmania aethiopica in a permissive monocytic cell line (THPl). Interferon-y (IFN-y), was one of the cytokines responsible for this anti-leishmania1 effect. IFN-y was necessary for inhibition but could not act on its own in inhibiting L. aethiopica. Tumor necrosis factor-a seemed not to be involved in the anti-L. aethiopica effect. The observed effects were in the absence of endotoxin.The results suggest that the mechanisms of killing of L. aethiopica in human cells may differ from those responsible for inhibition of other Leishmania parasites (such as Leishmania major) in mouse macrophages. Furthermore, that potentially relevant responses to Leishmania antigens may exist in normal individuals.