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Mechanisms associated with abnormal E-cadherin immunoreactivity in human bladder tumors

✍ Scribed by Pierre-Paul Bringuier; Laurence A. Giroldi; Rainy Umbas; Toru Shimazui; Jack A. Schalken

Publisher
John Wiley and Sons
Year
1999
Tongue
French
Weight
147 KB
Volume
83
Category
Article
ISSN
0020-7136

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✦ Synopsis

The involvement of E-cadherin in the progression of carcinoma is supported by a large number of studies showing an inverse relationship between E-cadherin immunoreactivity and tumor aggressiveness. However, the mechanisms leading to decreased E-cadherin immunoreactivity are still unclear. Comparison of Northern blotting and immunohistochemistry in a series of 49 frozen bladder tumors revealed that, in 16 of 23 tumors with abnormal staining, clear mRNA downregulation occurred. In the 7 cases without mRNA downregulation, no structural anomalies of E-cadherin could be detected by Southern blotting, Western blotting or PCR-SSCP. Western blotting confirmed that, in 6 of these tumors, E-cadherin was down-regulated at the protein level. This down-regulation was accompanied by down-regulation of ␣-catenin and, to a lesser extent, of ␤or ␥-catenin. However, Northern-blot analysis indicated that expression of the 3 catenins is maintained at the mRNA level. Thus our data show that, in bladder tumors, mRNA down-regulation accounts for about two thirds (16/23) of tumors with abnormal staining and that post-transcriptional down-regulation of E-cadherin occurs in 6/23 of these tumors. Int.

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