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Mechanism of ricin action on a wheat germ cell-free protein synthesizing system

✍ Scribed by Lugnier, Alain-AndréJean; Rether, Benoît

Book ID
122484479
Publisher
Elsevier Science
Year
1981
Weight
543 KB
Volume
23
Category
Article
ISSN
0304-4211

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A method is described for the preparation of a tRNA-dependent wheat germ protein-synthesizing system. This system can be supplied with exogenous tRNAs of eukaryotic or prokaryotic origin. In order to obtain maximal aminoacylation of the added tRNAs, the translation assays can be supplemented with ho