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Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity

โœ Scribed by Ruomei Gao; Zhuobin Yuan; Zhiqiang Zhao; Xiurui Gao


Book ID
108431250
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
157 KB
Volume
45
Category
Article
ISSN
1567-5394

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A new spectrophotometric assay of superoxide dismutase (SOD) activity is described. The assay is based on the SOD-mediated increase in the rate of autoxidation of 5,6,6a,11b-tetrahydro-3,9,10-trihydroxybenzo[c]fluorene (BXT-01050) in aqueous alkaline solution. This autoxidation yields a chromophore