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Mechanism of Methylene Blue Bleaching by Lipoxygenase

✍ Scribed by Toyosaki, Toshiyuki

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
349 KB
Volume
70
Category
Article
ISSN
0022-5142

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✦ Synopsis

A lipid peroxidation reaction and methylene blue bleaching reaction are thought to proceed in the presence of methylene blue with linoleic acid as the substrate from the time lipoxygenase is added until methylene blue bleaching concludes. For 15 s after adding the lipoxygenase, neither the lipid peroxidation reaction nor the bleaching reaction occurred, but they both proceeded after that time period. The results suggested that hydroperoxide is first formed by the lipid peroxidation reaction, after which methylene blue specifically picks the hydrogen from the hydroperoxide 1 3 -0 0 H isomers, and then the methylene blue is reduced.

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