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Mechanism of initiation of in vitro DNA synthesis by the immunopurified complex between yeast DNA polymerase I and DNA primase

โœ Scribed by Gianfranco BADARACCO; Paola VALSASNINI; Marco FOIANI; Roberta BENFANTE; Giovanna LUCCHINI; Paolo PLEVANI

Book ID
115124805
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
850 KB
Volume
161
Category
Article
ISSN
1432-1327

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Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperaturesensitive mutant altered in DNA primase-polymerase complex stability
โœ Lucchini, Giovanna ;Mazza, Cinzia ;Scacheri, Emanuela ;Plevani, Paolo ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer ๐ŸŒ English โš– 831 KB

The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Ph