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Mechanism of illegitimate recombination: Common sites for recombination and cleavage mediated by E. coli DNA gyrase

โœ Scribed by Ikeda, Hideo ;Kawasaki, Ichiro ;Gellert, Martin

Book ID
104772465
Publisher
Springer
Year
1984
Tongue
English
Weight
391 KB
Volume
196
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Illegitimate recombination dependent on D N A gyrase in a cell-free system has previously been described.

We have now mapped DNA gyrase cleavage sites in the vicinity of known recombination sites in pBR322. Among five recombination sites examined, three were found to coincide with a D N A gyrase cleavage site. This result suggests that the cleavage of D N A by DNA gyrase has a central role in the recombination process.

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