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Mechanism of endogenous phosphorylation of microtubule proteins during GTP-induced microtubule assembly and implications for stability of the assembled structures

✍ Scribed by Rosario Palomares; Veena Prasad; Richard Froilan Luduena; Rafael Manso-Martínez


Book ID
119120488
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
660 KB
Volume
927
Category
Article
ISSN
0167-4889

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Microtubule-associated proteins (MAPs) were separated from tubulin with several different methods. The ability of the isolated MAPs to reinduce assembly of phosphocellulose purified tubulin differed markedly between the different methods. MAPs isolated by addition of 0.35 M NaCl to taxol-stabilized