The most efficient method for polyphenol oxidase (PPO) extraction from Highbush blueberry fruits was the preparation of an acetone powder. No activity was detected after direct extraction with phosphate bu †er (pH 6É5) and detergents such as Triton X-100. PPO has been puriÐed 19-fold, by ultraÐltrat
Mechanism of Browning in Fresh Highbush Blueberry Fruit (Vaccinium corymbosum L). Role of Blueberry Polyphenol Oxidase, Chlorogenic Acid and Anthocyanins
✍ Scribed by Kader, Farid; Rovel, Bernard; Girardin, Michel; Metche, Maurice
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 216 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Browning reactions which occurred after crushing fresh blueberries (V accinium corymbosum L) were followed by determination of chlorogenic acid colour density, polymer colour and the level of polymers. A polyphenol oxidase (PPO) activity plays a dominant role in enzymatic browning. Peroxidase (POD) does not contribute to brown colour formation. The loss of 29% of the colour in a model system containing PPO and blueberry anthocyanins indicated that PPO could act directly on these pigments. However, the rate of anthocyanin degradation was stimulated by the addition of chlorogenic acid.
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