Continuous Assay for VanX, the d-Alanyl–
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Jeffrey J. Brandt; Lisa L. Chatwood; Ke-Wu Yang; Michael W. Crowder
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Article
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1999
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Elsevier Science
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English
⚖ 97 KB
The reaction of L-alanine-p-nitroanilide with VanX was studied in an effort to develop a continuous assay for VanX activity for future kinetic and inhibition studies. VanX, containing Zn(II), Co(II), Fe(II), or Ni(II), catalyzes the hydrolysis of L-alanine-p-nitroani- lide producing L-alanine and p-