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Mechanism-Based Inactivation of VanX, a d -Alanyl- d -alanine Dipeptidase Necessary for Vancomycin Resistance †

✍ Scribed by Aráoz, Romulo; Anhalt, Elizabeth; René, Loïc; Badet-Denisot, Marie-Ange; Courvalin, Patrice; Badet, Bernard


Book ID
125844934
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
102 KB
Volume
39
Category
Article
ISSN
0006-2960

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Continuous Assay for VanX, the d-Alanyl–
✍ Jeffrey J. Brandt; Lisa L. Chatwood; Ke-Wu Yang; Michael W. Crowder 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 97 KB

The reaction of L-alanine-p-nitroanilide with VanX was studied in an effort to develop a continuous assay for VanX activity for future kinetic and inhibition studies. VanX, containing Zn(II), Co(II), Fe(II), or Ni(II), catalyzes the hydrolysis of L-alanine-p-nitroani- lide producing L-alanine and p-