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Measuring the blockade of malaria transmission – An analysis of the Standard Membrane Feeding Assay

✍ Scribed by Thomas S. Churcher; Andrew M. Blagborough; Michael Delves; Chandra Ramakrishnan; Melissa C. Kapulu; Andrew R. Williams; Sumi Biswas; Dari F. Da; Anna Cohuet; Robert E. Sinden

Book ID
118454186
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
1010 KB
Volume
42
Category
Article
ISSN
0020-7519

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The internalization of fluorescent 7-nitro-2,1,3-benzoxadiazol-4-yl (NBD)-phospholipids from the plasma membrane can be assessed by the irreversible quenching of analogues in the outer leaflet by dithionite. Here we have utilized this assay to follow the redistribution of short-chain C6-NBD-sphingom