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Measuring growth of a phenanthrene-degrading bacterial inoculum in soil with a quantitative competitive polymerase chain reaction method

✍ Scribed by Egbert Schwartz; Sinh V. Trinh; Kate M. Scow

Book ID
109302333
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
272 KB
Volume
34
Category
Article
ISSN
0168-6496

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## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RT‐)PCR method is very useful. For this method, Cy5‐labeled primers were used, and after gel electrophoresis in 7β€…M urea, the Cy5‐labeled single‐stran