Measuring Calcium and Calmodulin Inside and Outside Cells

✍ Scribed by Emil C. Toescu, Alexej Verkhratsky (auth.), Ole H. Petersen MD MAE FMedSci, FRS (eds.)

Publisher: Springer-Verlag Berlin Heidelberg
Year: 2001
Tongue: English
Leaves: 301
Series: Springer Lab Manuals
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signalling molecules as well as very many pathophysiological processes including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles and in the immediate surroundings of individual cells are described in practical details by experts, on the basis of their personal experience. Considering that the protein calmodulin is the main target for Ca2+ action in most cells types, a chapter on tracking calmodulin inside cells, using fluorescence technology, has also been included.

✦ Table of Contents

Front Matter....Pages I-IX
Front Matter....Pages 1-1
Principles of Fluorescence Measurements — Dyes and Hardware Required....Pages 3-45
Simple Principles of Imaging — Image Files, Image Processing and Image Analysis....Pages 47-64
Aequorin Measurements of Cytosolic Ca 2+ ....Pages 65-89
Electrophysiological Measurements Using Ca 2+ -Sensitive Microelectrodes....Pages 91-102
Front Matter....Pages 103-103
Measuring Ca 2+ in the Nucleoplasm of Intact Cells....Pages 105-130
Measuring Ca 2+ in Isolated Nuclei....Pages 131-145
Ca 2+ Measurement with Luminescent Probes in the Endoplasmic Reticulum....Pages 147-163
Ca 2+ Measurement with Fluorescent Probes in the Endoplasmic Reticulum....Pages 165-185
Ca 2+ Measurements in Mitochondria....Pages 187-211
Ca 2+ Measurements in Secretory Granules....Pages 213-229
Ca 2+ Measurement in Endosomes of Intact Cells....Pages 231-247
Front Matter....Pages 249-249
Measuring Ca 2+ Extrusion from Single Cells....Pages 251-266
Tracking Calmodulin Inside Cells....Pages 267-290
Back Matter....Pages 291-301

✦ Subjects

Biochemistry, general; Human Physiology; Cell Biology

📜 SIMILAR VOLUMES

Calcium and Calmodulin Function in the C

📁 Calcium and Calmodulin Function in the Cell Nucleus

✍ Oriol Bachs Ph.D., Neus Agell Ph.D. (auth.) 📂 Library 📅 1995 🏛 Springer-Verlag Berlin Heidelberg 🌐 English

<p>This book is about the role of calcium and calmodulin in the cell nucleus. Calcium, which is an important second messenger of signal transduction pathways, can also operate in the cell nucleus. Different calcium binding proteins, which are the targets of cellular calcium, have been identified in

Cellular Regulators Part A: Calcium- and

📁 Cellular Regulators Part A: Calcium- and Calmodulin-Binding Proteins

✍ Anthony R. Means, P. Michael Conn (Eds.) 📂 Library 📅 1987 🏛 Academic Press 🌐 English

The critically acclaimed laboratory standard, <b>Methods in Enzymology</b>, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much

Cellular Regulators Part A: Calcium- and

📁 Cellular Regulators Part A: Calcium- and Calmodulin-Binding Proteins

✍ Anthony R. Means, P. Michael Conn (Eds.) 📂 Library 📅 1987 🏛 Academic Press 🌐 English

Inside and Outside Liquidity

📁 Inside and Outside Liquidity

✍ Bengt Holmstrom, Jean Tirole 📂 Library 📅 2011 🏛 The MIT Press 🌐 English

Two leading economists develop a theory explaining the demand for and supply of liquid assets.

Inside and Outside Liquidity

📁 Inside and Outside Liquidity

✍ Holmström, Bengt;Tirole, Jean 📂 Library 📅 2011;2013 🏛 MIT Press (MA) 🌐 English

Why do financial institutions, industrial companies, and households hold low-yielding money balances, Treasury bills, and other liquid assets? When and to what extent can the state and international financial markets make up for a shortage of liquid assets, allowing agents to save and share risk mor

Inside and Outside Liquidity

📁 Inside and Outside Liquidity

✍ Bengt Holmström, Jean Tirole 📂 Library 📅 2013 🏛 The MIT Press 🌐 English

<P>Why do financial institutions, industrial companies, and households hold low-yielding money balances, Treasury bills, and other liquid assets? When and to what extent can the state and international financial markets make up for a shortage of liquid